Glycerol can be used in FDA-approved wound and burn treatments, bacterial culture media, as an effective biomarker for assessing liver disease, as a sweetener in the food industry, and as a humectant in pharmaceutical formulations. This dataset includes primary raw materials, auxiliary materials, energy inputs, and atmospheric emissions associated with the production process; however, infrastructure such as production equipment and facility construction is excluded from the system boundary. Life cycle inventory data were sourced from literature and optimized based on the best available information for 2022. The process of producing biodiesel from collected, treated, and processed waste edible oils includes filtration and pretreatment, esterification, neutralization, separation, decolorization and purification, water and acid removal, and distillation. and refining processes, where moisture is evaporated through high-temperature cooking to separate impurities and water from the oil, with glycerol as a co-product.