Glycerol can be used in FDA-approved wound and burn treatments, bacterial culture media, as an effective biomarker for liver disease, as a sweetener in the food industry, and as a humectant in pharmaceutical formulations; this dataset includes primary raw and auxiliary materials, energy consumption, atmospheric emissions, and waste from the production process, while infrastructure such as production equipment and facility construction is excluded from the system boundary; the life cycle inventory data were sourced from literature and optimized based on the best available information for 2022; the process of producing biodiesel from animal fats includes steps such as fat extraction, impurity removal, transesterification, separation, and post-treatment, with glycerol being a co-product. Product under production.