Glycerol can be used in FDA-approved wound and burn treatments, bacterial culture media, an effective biomarker for assessing liver disease, a sweetener in the food industry, and a humectant in pharmaceutical formulations. This dataset includes primary raw materials, energy inputs, and atmospheric emissions from the production process; however, infrastructure such as production equipment and facility construction are excluded from the system boundary. The life cycle inventory data were sourced from the literature and optimized based on the best available information from 2022. The process flow for producing biodiesel from waste edible oil collection, treatment, and processing includes filtration and pretreatment, esterification, neutralization, separation, decolorization and purification, water and acid removal, and distillation. , refining and other processes, where impurities and water in the oil are separated by heating and then allowing sedimentation, with glycerol as a co-product.