Glycerol can be used in FDA-approved wound and burn treatments, bacterial culture media, as an effective biomarker for liver disease, as a sweetener in the food industry, and as a humectant in pharmaceutical formulations; this dataset includes primary raw and auxiliary materials, energy inputs, and atmospheric emissions from the production process, but infrastructure such as production equipment and facility construction is not included within the system boundary; the life cycle inventory data were sourced from literature and optimized based on the best available information for 2022; the process flow for producing biodiesel from vegetable oil includes pretreatment, esterification, neutralization, separation and purification, and distillation, with glycerol being a co-product.